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Identification and characterization of the n ‐6 fatty acid‐producing Mucor rouxii native isolate CFR‐G15
Author(s) -
Mamatha Shivaramu S.,
Halami Prakash M.,
Venkateswaran Govindarajulu
Publication year - 2010
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200900101
Subject(s) - mycelium , biology , mucor , microbiology and biotechnology , botany , aspergillus
In zygomycetes fungi, many Mucor spp. have been known to produce γ‐linolenic acid (GLA) in their biomass. Among 250 soil samples screened, 20 Mucor isolates showed GLA in their mycelial mass under normal cultivation conditions. Sudan Black B was used for screening their qualitative oleaginesity. Among the representative isolates, Mucor sp. CFR‐G15, when grown in a fat‐producing medium, showed a maximum lipid content of 30 ± 1.32% in its mycelia and 14.42 ± 0.74% GLA. By using gene‐specific primers, the 18S rRNA gene and the Δ 6 DES gene were amplified by PCR technique. The nucleotide sequences of the 18S rRNA and Δ 6 DES genes exhibited >98% homology with M. rouxii ATCC 24905 (accession nos. AF117923 and AF296076, respectively), suggesting taxonomic identity. The native isolate M. rouxii CFR‐G15 reported in this study was found to be promising for the development of an economical process in the industrial production of GLA.