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Improvement of Staphylococcus xylosus lipase production through optimization of the culture conditions
Author(s) -
Ghribi Dhouha,
Sayari Adel,
Gargouri Youssef,
Bezzine Sofiane
Publication year - 2009
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200900004
Subject(s) - lipase , staphylococcus xylosus , chemistry , food science , yeast extract , casein , fermentation , aeration , biochemistry , ammonium sulfate , chymosin , chromatography , staphylococcus , enzyme , biology , staphylococcus aureus , bacteria , organic chemistry , genetics
The Staphylococcus xylosus strain produces without induction an original lipase named S. xylosus lipase (SXL). Since considerable interest has been given to microbial lipases for biotechnology applications like detergents, food, drugs and pharmaceutical products, improvement of their production is of great importance to reduce the final cost. This goal could be reached through the optimization of several physicochemical culture conditions. Indeed, an appropriate medium was formulated for SXL production. It was composed of 17 g/L pancreatic digest of casein, 2.5 g/L glucose, 6 g/L yeast extract, 0.75 g/L ammonium sulfate corresponding to a C/N ratio of 6, 1 g/L K 2 HPO 4 and 1 g/L KH 2 PO 4 . In such a medium, SXL production reached 42 U/mL. Moreover, the usefulness of such a medium for large‐scale production of SXL was also evidenced in an automated fully controlled 2.6‐L fermenter. It was shown that aeration of the medium, which strongly affected the growth, regulated the lipase synthesis by the produced cells. It was found that when using a dissolved oxygen saturation of the medium of 50%, the SXL production reached 62 U/mL.