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Oxidation products of free polyunsaturated fatty acids in wheat varieties
Author(s) -
Levandi Tuuli,
Püssa Tõnu,
Vaher Merike,
Toomik Peeter,
Kaljurand Mihkel
Publication year - 2009
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200800286
Subject(s) - chemistry , polyunsaturated fatty acid , formic acid , electrospray ionization , chromatography , linoleic acid , tandem mass spectrometry , high performance liquid chromatography , mass spectrometry , fatty acid , biochemistry
Oxygenated fatty acids (oxylipins) are secondary metabolites of polyunsaturated fatty acids (PUFA). Here, we present a novel high‐performance liquid chromatograpic separation on a reversed‐phase column (RP‐HPLC) coupled with electrospray ionization‐tandem mass spectrometry (ESI‐MS/MS) for the determination of various (per)oxidation products of linoleic ( cis , cis ‐9,12‐octadecadienoic) acid in eight different varieties (four spring and four winter varieties) of wheat ( Triticum aestivum ). The procedure includes extraction of oxylipins, chromatograpic separation using a linear gradient of aqueous formic acid and acetonitrile, with subsequent identification of compounds by MS/MS. Among the identified oxylipins, leukotoxin (LTX)‐diol and its isomer (iso‐LTX‐diol) are known as potentially toxic substances. The obtained data was used further for comparison of different wheat varieties by principal component analysis (PCA). From the results of PCA, differences can be observed in the patterns of wheat varieties.