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Separation of squalene from palm fatty acid distillate using adsorption chromatography
Author(s) -
Chua Camilla Soo Leng,
Baharin Badlishah Sham,
Man Yaakob B. Che,
Tan Chin Ping
Publication year - 2007
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200700312
Subject(s) - squalene , chemistry , chromatography , hydrolysis , neutralization , adsorption , column chromatography , distillation , fatty acid , lipase , acid hydrolysis , hexane , organic chemistry , antibody , immunology , biology , enzyme
A method to separate squalene from palm fatty acid distillate (PFAD) using neutralization‐hydrolysis‐neutralization before employing adsorption column chromatography was developed. Extraneous matters, especially free fatty acids (83.8%) and acylglycerols (12.7%), were first neutralized and removed before being subjected to hydrolysis by using commercially available, immobilized Candida antartica lipase, at 65 °C for 8 h. Neutralization followed by hydrolysis and repeated neutralization successfully concentrated squalene from an initial amount of 3.76% to 27.5%. Oil extracted from neutralized‐hydrolyzed‐neutralized PFAD was then passed through a Diaion HP‐20 column using reverse‐phase adsorption chromatography. Squalene was desorbed by hexane, with a recovery of 93%.