Premium
Conjugated linoleic acid (CLA) production and lipase‐catalyzed interesterification of purified CLA with canola oil
Author(s) -
Goli Sayyed Amir Hossein,
Kadivar Mahdi,
Keramat Javad,
Fazilati Mohammad
Publication year - 2008
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200700267
Subject(s) - canola , interesterified fat , chemistry , lipase , rapeseed , urea , conjugated linoleic acid , food science , oleic acid , organic chemistry , linoleic acid , fatty acid , chromatography , biochemistry , enzyme
In this study, two important isomers of CLA, i.e. c 9, t 11 and t 10, c 12, were produced up to ca. 73% of total fatty acids, employing alkali isomerization of safflower oil, followed by purification with only one‐step urea crystallization to 85.6%, while the recovery of the purification process was 35%. Interesterification (acidolysis) of purified CLA with canola oil was then conducted by Thermomyces lanuginosus lipase. The CLA content incorporated into the triacylglycerols (TG) was 26.6 mol‐% after 48 h of reaction time. Physical and chemical properties of the TG were then changed according to the degree of substitution of oleic acid in canola oil with CLA.