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Comparison of extraction methods for determining tocopherols in soybeans
Author(s) -
Lim Ho,
Woo Sunhee,
Kim Hong Sig,
Jong SeungKeun,
Lee Junsoo
Publication year - 2007
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200700060
Subject(s) - saponification , chromatography , extraction (chemistry) , chemistry , tocopherol , high performance liquid chromatography , vitamin e , antioxidant , organic chemistry
We evaluated the usefulness of saponification, direct solvent extraction, and Soxhlet extraction as extraction methods to determine the amounts of tocopherols in soybeans. Soxhlet extraction yielded the highest analytical values for each tocopherol homolog and was the best method for quantifying tocopherols in soybeans. Coupling of simple Soxhlet extraction with HPLC provided a highly reproducible procedure to quantify tocopherols in soybeans. The percent mean recovery ± standard deviation ( n = 5) was 103.2 ± 1.21, 109.8 ± 4.18, 93.8 ± 1.12, and 106.9 ± 1.54% for α‐, β‐, γ‐, and δ‐tocopherol, respectively. The linearity test for quantification was carried out over the ranges of 0.4–10.0, 0.2–4.0, 2.0–16.0, and 0.4–10.0 µg/mL for α‐, β‐, γ‐, and δ‐tocopherol, respectively. Regression analysis showed an excellent linear relationship ( R 2 >0.998), and the results of the validation parameters were generally reliable and satisfactory.