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Stereospecific analysis of phospholipid classes in rat muscle
Author(s) -
PérezPalacios Trinidad,
Antequera Teresa,
Muriel Elena,
Ruiz Jorge
Publication year - 2006
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200600104
Subject(s) - phospholipid , phosphatidylethanolamine , polyunsaturated fatty acid , docosahexaenoic acid , palmitic acid , chemistry , phosphatidylcholine , fatty acid , arachidonic acid , linoleic acid , oleic acid , stearic acid , phosphatidylserine , phosphatidylinositol , biochemistry , stereochemistry , organic chemistry , membrane , kinase , enzyme
The fatty acid and dimethyl acetal (DMA) compositions of the sn‐ 1 and the sn‐ 2 positions from individual phospholipid (PL) classes [phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI)], obtained from the Longissimus dorsi muscle of rat were studied. The profile of fatty acids and DMA in the sn‐ 1 and sn‐ 2 positions of all the PL classes showed marked differences. Overall, saturated fatty acids and monounsaturated fatty acids were in a higher proportion in the sn‐ 1 position than in the sn‐ 2 one, while polyunsaturated fatty acids were mainly in the sn‐ 2 position. Significant differences in the DMA and fatty acid compositions between individual PL classes for each position were found. PC contained the highest proportion of palmitic acid (16:0) in the sn‐ 1 position and of linoleic acid (18:2 n‐ 6) in the sn‐ 2 one. The highest values for stearic acid (18:0) and DMA in the sn‐ 1 position and for docosahexaenoic acid (22:6 n‐ 3) in the sn‐ 2 position were found in PE. PS and PI showed the highest proportion of oleic acid (18:1 n‐ 9) in the sn‐ 1 position and of arachidonic acid (20:4 n‐ 6) in the sn‐ 2 position.