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Enzyme‐catalyzed synthesis of structured phospholipids with conjugated linoleic acid
Author(s) -
Hossen Monjur,
Hernandez Ernesto
Publication year - 2005
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200501190
Subject(s) - lipase , conjugated linoleic acid , chemistry , enzyme , phospholipase a2 , phospholipase , phospholipase a1 , linoleic acid , biochemistry , fatty acid , conjugated system , catalysis , organic chemistry , chromatography , polymer
Structured phospholipids were synthesized with the functional lipid conjugated linoleic acid (CLA). The lipase‐ and phospholipase A 2 ‐catalyzed enzymatic acidolysis reaction between phospholipids (PL) and CLA was used for fatty acid modification. Enzymatic processes were an effective way to produce structured PL. Screening of four lipases and immobilized phospholipase A 2 and a combination of lipase and phospholipase showed that only Lipozyme RM IM and Lipozyme TL IM were effective in incorporation of CLA into PL. The maximum incorporation achieved by the latter enzyme was 16% with soy PL in 72 h.