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Elaidic acid in rat liver identified by gas chromatography and nuclear magnetic resonance spectroscopy
Author(s) -
Sabarense Céphora Maria,
de Almeida Gonçalves Maria Inês,
Filho Jorge Mancini
Publication year - 2006
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200500300
Subject(s) - elaidic acid , gas chromatography , chemistry , fatty acid , nuclear magnetic resonance spectroscopy , chromatography , spectroscopy , organic chemistry , linoleic acid , physics , quantum mechanics
Fatty acid composition has been commonly determined by gas chromatography (GC). In the most commonly used methods, the lipids must be converted into methyl esters or other derivatives of the fatty acids before being analyzed by GC. Nuclear magnetic resonance (NMR) spectroscopy has become one of the most promising methods to determine organic structures, providing useful data for analyzing the fatty acid composition of edible vegetable oils. The major advantage of NMR is that identification of each fatty acid is carried out by evaluation of particular signals. We report in this work the fatty acid profile of rat liver after consumption of diets containing different concentrations of partially hydrogenated vegetable fat. The fatty acid composition was identified by GC, and trans fatty acids were also identified by 13 C NMR spectroscopy. The results obtained by the 13 C NMR method were close enough to those obtained by conventional GC.