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Bis ‐methylene‐interrupted octadecadienoic fatty acids in Bulgarian bovine butter fats
Author(s) -
Nikolova Diana,
Antonova Daniela,
Marekov Ilko,
NikolovaDamyanova Boryana
Publication year - 2006
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200500297
Subject(s) - chemistry , double bond , methylene , mass spectrometry , fatty acid , bovine milk , silver nitrate , gas chromatography , chromatography , organic chemistry
Preparative silver nitrate thin‐layer chromatography of fatty acid methyl esters and gas chromatography‐mass spectrometry of 4,4‐dimethyloxazoline derivatives were applied in combination to analyze the cis , cis octadecadienoic (18:2) fraction in commercial samples of bovine butter fat in Bulgaria. The main component, 9,12–18:2 was accompanied by a series of bis ‐methylene‐interrupted isomers, cis , cis ‐5,9‐; 6,10‐; 9,13‐ and 11,15–18:2, which were unambiguously identified by their specific mass spectra. The latter are characterized by the presence of very intensive diagnostic fragments which help to correctly locate the position of the double bond system in the carbon chain. The cis , cis ‐5,9–18:2 isomer was found for the first time in butter fat, and the 6,10–18:2 isomer has not been described as yet.