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A micro‐method for lipoprotein cholesterol profiles: Impact of CETP in KKA y mice
Author(s) -
Alberts Pe¯teris,
Klingström Gunnel,
ArrheniusNyberg Vibeke,
Larsson Catarina,
Sakariassen Kjell S.
Publication year - 2006
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200500261
Subject(s) - cholesterylester transfer protein , very low density lipoprotein , lipoprotein , cholesterol , genetically modified mouse , medicine , hamster , endocrinology , transgene , high density lipoprotein , chemistry , intermediate density lipoprotein , biology , biochemistry , gene
The aim of the present study was to assess cholesterol‐containing lipoprotein profiles in minute serum samples. The lipoprotein profiles of KKA y and transgenic KKA y ‐CETP mice and of other species were determined. The transgenic KKA y ‐CETP mice express the simian enzyme cholesteryl ester transfer protein (CETP). The serum profile of the cholesterol‐containing high‐density (HDL), low‐density (LDL) and very‐low‐density lipoproteins (VLDL) was monitored on a Superose 6 column using fast protein liquid chromatography. Serum from several mouse and rat strains, rabbit, hamster, pig and man was included for comparative and method validation purposes. The chromatograms showed that the transgenic KKA y ‐CETP mice had significantly decreased relative levels of HDL vs . VLDL and LDL cholesterol ( p <0.001). Introduction of the CETP gene shifted the serum profile of the cholesterol‐containing lipoproteins of the KKA y ‐CETP mice closer to the human profile in a dose‐dependent manner, thus making these mice an interesting model for man. The described lipoprotein separation technology offers promising and reliable opportunities for studies of blood lipoprotein profiles with minute serum samples, in both animals and man.