Nematicidal sphingolipids from the freshwater fungus Paraniesslia sp. YMF1.01400

A new ( 1 ) and a known ( 2 ) sphingolipid were isolated from mycelial cultures of Paraniesslia sp. YMF1.01400. The structures of these two isolates were elucidated as (2 S ,2' R ,3 R ,3' E ,4 E ,8 E )‐1‐O‐(β‐ D ‐glucopyranosyl)‐3‐hydroxyl‐2‐[N‐2'‐hydroxyl‐3'‐eico‐sadecenoyl]amino‐9‐methyl‐4,8‐octadecadiene ( 1 ) and (2 S ,2' R ,3 R ,3' E ,4 E ,8 E )‐1‐O‐(β‐ D ‐glucopyranosyl)‐3‐hydroxyl‐2‐[N‐2'‐hydroxyl‐3'‐octadecenoyl]amino‐9‐methyl‐4,8‐octadecadiene ( 2 ) by spectroscopic and chemical methods. In in vitro tests, both compounds showed moderate nematicidal activities against Bursaphelenchus xylophilus . This is the first report of secondary metabolites in a genus Paraniesslia aquatic fungus.