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Downstream processing of mannosylerythritol lipids produced by Pseudozyma aphidis
Author(s) -
Rau Udo,
Nguyen La Anh,
Roeper Harald,
Koch Helmut,
Lang Siegmund
Publication year - 2005
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200401122
Subject(s) - amberlite , chromatography , downstream processing , chemistry , adsorption , pulmonary surfactant , suspension (topology) , bioreactor , solvent , organic chemistry , biochemistry , mathematics , homotopy , pure mathematics
Pseudozyma aphidis DSM 14930 was used for the bioreactor production of mannosylerythritol lipids (MEL). A scheme is presented for the isolation and purification of MEL by using different solvents. Adsorption experiments of MEL at Amberlite XAD resins were performed. Up to 93% (wt‐%) MEL could be transferred from the culture suspension into a newly formed, highly viscous solid phase by heating the culture suspension to ≥100 °C. This phase contained on average 87% (wt‐%) MEL and could be simply isolated by pouring off the supernatant, without producing solvent waste.