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Antioxidant activity of evening primrose phenolics in sunflower and rapeseed oils
Author(s) -
Schmidt Štefan,
Niklová Ivana,
Pokorný Jan,
Farkaš Pavel,
Sekretár Stanislav
Publication year - 2003
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200300842
Subject(s) - evening primrose , chemistry , gallic acid , antioxidant , food science , ethyl acetate , rapeseed , sunflower , protocatechuic acid , chromatography , catechin , polyphenol , organic chemistry , biology , horticulture
Crude ethanol/ethyl acetate extracts of industrial evening primrose ( Oenothera biennis L.) seed meal were separated into six fractions using the Sephadex LH‐20 column chromatography and 96% aqueous ethanol as a mobile phase. Their antioxidant activities were tested in sunflower and rapeseed oils by using an Oxidograph apparatus at a temperature of 110 °C. Only the fractions III and IV displayed a pronounced antioxidant activity while the other fractions were either inactive or even pro‐oxidative. The active fractions contained phenolic acids and their esters; gallic acid, methyl and ethyl gallates, protocatechuic acid and its methyl ester were identified by GC/MS. Catechin was present, too, but exhibited only moderate antioxidant activity in sunflower oil.