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Determination and certification of the relative mass fractions of triglycerides and cholesterol in pure and adulterated butter oil
Author(s) -
Zeleny Reinhard,
Schimmel Heinz
Publication year - 2003
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200300824
Subject(s) - repeatability , chromatography , chemistry , calibration , coconut oil , certified reference materials , butterfat , mathematics , analytical chemistry (journal) , food science , detection limit , statistics , milk fat , linseed oil
Pure butter oil and adulterated butter oil (5% coconut oil added) were certified for the relative mass fractions of triglycerides C 24 ‐C 54 and cholesterol by employing a reference method defined in EC‐regulation 454/95 based on gas chromatographic separation of the triglycerides on packed or capillary columns. Four data sets each of packed‐column and capillary‐column methods were accepted and utilised to establish the certified values. Technical evaluation of the data revealed that calibration is a critical issue in terms of composition of the calibrant and possible precipitation. Recalculations of submitted data were carried out, which mainly involved linear interpolation to calculate the response factors of components not present in the calibrant. This procedure decreased the overall variation of results. Data derived from capillary‐column methods differing in column length, diameter, coating, and temperature program were found to be comparable to those obtained by the more uniform packed‐column methods as regards precision, repeatability, and obtained S‐values. The presented results shall provide a basis to further refine the existing reference method.