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An integrated microbial/enzymatic process for production of rhamnolipids and L‐(+)‐rhamnose from rapeseed oil with Pseudomonas sp. DSM 2874
Author(s) -
Trummler Klaus,
Effenberger Franz,
Syldatk Christoph
Publication year - 2003
Publication title -
european journal of lipid science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 94
eISSN - 1438-9312
pISSN - 1438-7697
DOI - 10.1002/ejlt.200300816
Subject(s) - rhamnolipid , rhamnose , chemistry , decanoic acid , rapeseed , pseudomonas putida , food science , chromatography , enzyme , biochemistry , mannose , bacteria , biology , genetics , pseudomonas aeruginosa
The object of this paper is the development of a biotechnological process for production of rhamnolipids by Pseudomoas sp. DSM 2874. The product obtained from rapeseed oil was found to be a mixture of up to four glycolipids (rhamnolipid 1 ‐ 4), L‐(+)rhamnose and ( R , R )‐3‐(3‐hydroxydecanoyloxy) decanoic acid. An HPLC‐method was developed for identification and quantification of rhamnolipids and fatty acids, using an evaporative light scattering detector. Growing cells under nitrogen limiting conditions and resting cells were used for rhamnolipid production with triglycerides as C‐source. Best results and product yields (up to 45 g l ‐1 of mixtures of rhamnolipid 1 and rhamnolipid 3) were obtained using resting cells in a fed batch process with rapeseed oil as C‐source. Product composition was found to be strongly dependent on the process conditions. Using the fungal enzyme Naringinase it was possible to cleave the glycosidic bond between the two rhamnose units of rhamnolipid 3, and the bond between L‐(+)‐rhamnose and ( R , R )‐3‐(3‐hydroxydecanoyloxy) decanoic acid of rhamnolipid 1. The latter reaction occurred at a much lower rate. Direct addition of Naringinase to resting cells in an integrated microbial/enzymatic process was possible and resulted in an alteration of product composition from a rhamnolipid 1 and rhamnolipid 3 mixture to rhamnolipid 1 only.