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Cell‐Membrane Staining Properties and Photocytotoxicity of a Ruthenium(II) Photosensitizer
Author(s) -
Zhang Kenneth Yin,
Song Linna,
Gu Tianhan,
Wang Hao,
Yang Chao,
Zhou Hanchen,
Gao Pengli,
Liu Shujuan,
Zhao Qiang
Publication year - 2020
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.202000702
Subject(s) - photosensitizer , chemistry , hela , photodynamic therapy , ruthenium , intracellular , reactive oxygen species , programmed cell death , organelle , biophysics , ruthenium red , cell membrane , cell , membrane , cell growth , phototoxicity , irradiation , photochemistry , membrane permeability , apoptosis , biochemistry , in vitro , calcium , organic chemistry , catalysis , biology , physics , nuclear physics
Organelle‐targeting photosensitizers have attracted increasing attention in the development of drugs for photodynamic therapy because they can efficiently induce cellular functional disorder upon light irradiation. In this work, we reported a ruthenium(II) complex bearing two lipophilic carbon chains which specifically binds to the cell membrane. Light irradiation on the complex‐treated cells caused an increase of intracellular reactive oxygen species. The photocytotoxicity of the complex toward HeLa cells were evaluated via the 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. A high dose (3 µ m ) of the complex induced apoptotic cell death by photogeneration of intracellular reactive oxygen species. A low dose (0.25 µ m ) of the complex did not cause noticeable cell death but increased the permeability of the cell membrane upon light irradiation.