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Azurin and HS – : Towards Implementation of a Sensor for HS – Detection
Author(s) -
Strianese Maria,
Palm Gottfried J.,
Kohlhause David,
Ndamba Lionel A.,
Tabares Leandro C.,
Pellecchia Claudio
Publication year - 2019
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201801399
Subject(s) - azurin , chemistry , bioinorganic chemistry , fluorescence , metalloprotein , quenching (fluorescence) , reactivity (psychology) , cobalt , electron paramagnetic resonance , photochemistry , crystallography , electron transfer , inorganic chemistry , stereochemistry , enzyme , nuclear magnetic resonance , organic chemistry , medicine , physics , alternative medicine , quantum mechanics , pathology
Hydrogen sulfide (H 2 S) is an important biological messenger, which recently joined nitric oxide (NO) and carbon monoxide (CO) as an endogenously produced gasotransmitter. This discovery resulted in a renewed interest towards H 2 S and, in particular, in its reactivity with biological and bioinorganic targets. However, in the scientific literature studies exploring the reactivity of H 2 S/HS – with non‐heme proteins are rare. In this work, we studied the interaction of HS – with the natural metalloprotein Cu‐azurin and its cobalt variant via UV‐Vis, fluorescence, and EPR spectroscopy, as well as X‐ray diffraction. Interaction of HS – significantly enhances the fluorescence emission of fluorescently labelled copper azurin in buffered solutions and at physiological pH. In contrast, fluorescently labelled cobalt azurin undergoes a fluorescence quenching in the presence of HS – in line with the changes observed in the absorption spectrum of the construct. The results highlight the potential of azurin for implementation in a fast and simple HS – sensor.