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Pyrophosphate Prompted Aggregation‐Induced Emission: Chemosensor Studies, Cell Imaging, Cytotoxicity, and Hydrolysis of the Phosphoester Bond with Alkaline Phosphatase
Author(s) -
Raj Pushap,
Singh Amanpreet,
Singh Ajnesh,
Singh Ashutosh,
Garg Neha,
Kaur Navneet,
Singh Narinder
Publication year - 2019
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201801173
Subject(s) - chemistry , pyrophosphate , phosphodiester bond , alkaline phosphatase , zinc , fluorescence , aqueous solution , stereochemistry , enzyme , organic chemistry , biochemistry , rna , physics , quantum mechanics , gene
Two benzimidazole‐based zinc complexes [(Zn) 2 (L2) 2 Cl 2 (DMSO) 2 ] ( R1 ) and [Zn(L2) 2 (NO 3 ) 2 (H 2 O) 2 ] ( R2 ) were synthesized and characterized with various spectroscopic data. The single X‐ray structure determination reveals that complex R1 is dinuclear and tetrahedral in geometry, while complex R2 is mononuclear and octahedral in geometry. Further, both zinc complexes were investigated for pyrophosphate sensing in an aqueous medium. Complex R1 is found to be selective towards pyrophosphate; it leads to 5.5‐fold enhancement in the emission intensity due to aggregation‐induced emission. However, complex R2 has shown binding with all, ATP, AMP, ADP, and pyrophosphate, which is attributed to the chelate effect. Consequently, complex R1 was utilized for the intracellular detection of pyrophosphate in HeLa cells. Furthermore, the PPi based zinc complex R1 is also used as a bio‐analytical tool to construct a real‐time fluorescence assay for the enzymatic activity of alkaline phosphatases (ALP).