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The Influence of Redox‐Active Transition Metal Containing Micro‐ and Nanoparticles on the Properties of Representative Bioinorganic Reaction Systems
Author(s) -
Đurović Mirjana,
Oszajca Maria,
Stochel Grażyna,
van Eldik Rudi
Publication year - 2018
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201701421
Subject(s) - chemistry , metmyoglobin , bioinorganic chemistry , myoglobin , nanoparticle , nanomaterials , transition metal , molecule , redox , biomolecule , metal , combinatorial chemistry , oxide , kinetics , catalysis , nanotechnology , inorganic chemistry , organic chemistry , biochemistry , materials science , physics , quantum mechanics
The use of nanomaterials in biological applications requires a better understanding of the effect of nanoparticles on the activity of proteins and related molecules. We have examined the influence of environmental particulate matter (PM) on the interaction of myoglobin and microperoxidase‐11 as model systems with the small molecules O 2 and NO, respectively. The influence of PM on the reaction of dioxygen with deoxymyoglobin to form oxymyoglobin, and subsequently metmyoglobin, was studied for its essential role in living organisms. Samples of urban PM (SRM 1648a) were used as standard reference material. Also the influence of Fe 2 O 3 nanoparticles on the stability of the investigated protein was studied. The obtained results show that binding and release of dioxygen by deoxymyoglobin may be affected by the presence of PM especially over longer periods of time. Similar studies were performed on the nitrosylation of microperoxidase‐11 as a model for the catalytic center of enzymes, for which the influence of the standard reference material, its water‐soluble extract, as well as metal oxide nanoparticles (Fe 2 O 3 and ZnO), were studied. The reported results show that the presence of PM had no influence on the kinetics of the nitrosylation of the model microperoxidase under the selected experimental conditions.