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Purine‐Based Fluorescent Sensors for Imaging Zinc Ions in HeLa Cells
Author(s) -
Singh Swati,
Sivakumar Sri,
Verma Sandeep
Publication year - 2017
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201700806
Subject(s) - chemistry , schiff base , hela , imine , fluorescence , zinc , purine , stereochemistry , biochemistry , organic chemistry , in vitro , physics , quantum mechanics , enzyme , catalysis
We synthesized three chelate‐tethered purine ligands, having a Schiff base linkage at the N9 position, which resulted in the dipolar imine‐methoxyphenol adenine Schiff base HL1 , the 2‐aminopurine Schiff base HL2 , and the 2,6‐diaminopurine Schiff base HL3 . These ligands selectively showed enhancement of the fluorescence of the zinc ions. The imine‐based compound HL1 chelated with zinc ions to give the discrete dimeric structure 1 , a green fluorescent complex, which was again stabilized by purine–purine interactions to give a 1D polymeric structure. The ligands having a 6‐amino group, HL1 and HL3 , have high quantum yield, which gives higher fluorescence enhancement of the free zinc ions in the HeLa cells. HL1 was localized in the cytoplasm and the nucleoli of the HeLa cells, which was confirmed by an RNase digestion test.