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Application of a Metal‐Mediated Base Pair to the Detection of Medicinally Relevant Single Nucleotide Polymorphisms
Author(s) -
Jash Biswarup,
Müller Jens
Publication year - 2017
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201700665
Subject(s) - nucleobase , chemistry , base pair , molecular beacon , thymine , oligonucleotide , phenanthroline , single nucleotide polymorphism , metal , nucleotide , cytosine , dna , combinatorial chemistry , stereochemistry , crystallography , gene , biochemistry , organic chemistry , genotype
A highly stabilizing metal‐mediated base pair formed from the canonical nucleobase cytosine (C), the transition metal ion Ag I , and the artificial nucleobase 1 H ‐imidazo[4,5‐ f ][1,10]phenanthroline (P) was used in the generation of molecular beacons for the detection of medicinally relevant single nucleotide polymorphisms (SNPs) based on either a C→T or a T→C transition (T: thymine). The beacons exploit the fact that a P–C pair is strongly stabilized in the presence of Ag I , whereas a P–T pair is significantly destabilized. A direct correlation of oligonucleotide sequence, duplex stability prior to the addition of Ag I , stabilization due to metal‐mediated base pair formation, and performance of the molecular beacon was not observed. Nonetheless, the data show for the first time that metal‐mediated base pairing can be applied in the detection of medicinally relevant SNPs.