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Anchoring Cytochrome b 562 on a Gold Nanoparticle by a Heme–Heme Pocket Interaction
Author(s) -
Onoda Akira,
Taniguchi Tomoaki,
Inoue Nozomu,
Kamii Ayumi,
Hayashi Takashi
Publication year - 2016
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201600301
Subject(s) - chemistry , moiety , colloidal gold , heme , hemeprotein , electrode , electrochemistry , dielectric spectroscopy , nanoparticle , cyclic voltammetry , stereochemistry , inorganic chemistry , nanotechnology , organic chemistry , materials science , enzyme
Gold nanoparticles immobilized on a gold electrode surface were modified with a heme moiety through gold–sulfur bonds and then treated with apocytochrome b 562 (apoCYT) to yield a reconstituted protein on the gold nanoparticles. Immobilization of apoCYT on the surface of the AuNP‐modified electrode was confirmed by the charge‐transfer resistance value R CT determined using alternating‐current electrochemical impedance spectroscopy. The N 1s and Fe 2p peaks in the X‐ray photoelectron spectrum support the reconstitution of the heme moiety on the surface of the AuNP electrode. The hemoprotein‐immobilized electrode exhibits a ten‐fold enhanced electrochemical response relative to the protein immobilized directly on the gold electrode.