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Receptor‐Targeted Luminescent Silver Bionanoparticles
Author(s) -
Bunschoten Anton,
Chin Patrick T. K.,
Buckle Tessa,
van der Linden Marte,
Barendregt Arjan,
Verheijen Marcel A.,
van Leeuwen Fijs W. B.
Publication year - 2016
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201501414
Subject(s) - nanoclusters , luminescence , chemistry , human serum albumin , nanoparticle , nanotechnology , biophysics , materials science , biochemistry , organic chemistry , optoelectronics , biology
Luminescent Ag nanoclusters (Ag‐NC) provide the next generation in bionanoparticles, wherein the luminescence (650 nm) and large Stokes shift of these inorganic nanoclusters are favorable for biological imaging. By combining these characteristics with those of human serum albumin (HSA; a protein capable of binding multiple endo‐ and exogenous compounds), the Ag nanoclusters can be shielded from the environment and functionalized with (receptor) targeting moieties. Encapsulation of the 1.5 nm Ag nanoclusters by HSA resulted in a threefold increase in luminescence intensity and a twofold increase of the luminescence lifetime (1.7 vs. 3.6 µs). To exemplify the potential of this targeted concept, we functionalized HSA‐Ag nanoparticles with chemokine receptor 4 (CXCR4) targeting peptides [Ac‐TZ14011(CO 2 H)]. The resulting Ac‐TZ14011‐HSA‐Ag nanoparticles demonstrated specific binding to CXCR4‐overexpressing tumor cells. Upon exposure to (ambient) light, particle‐functionalized tumor cells were killed. Combined, these experiments illustrate that HSA‐Ag nanoparticles may have a potential in biological imaging and possibly even in targeted theranostic applications.