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Ru II (η 6 ‐ p ‐cymene) Complexes of Bioactive 1,2‐Benzothiazines: Protein Binding vs. Antitumor Activity
Author(s) -
Ashraf Adnan,
Kubanik Mario,
Aman Farhana,
Holtkamp Hannah,
Söhnel Tilo,
Jamieson Stephen M. F.,
Hanif Muhammad,
Siddiqui Waseeq Ahmad,
Hartinger Christian G.
Publication year - 2016
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201501361
Subject(s) - chemistry , benzothiazine , stereochemistry , context (archaeology) , ligand (biochemistry) , reactivity (psychology) , biological activity , in vitro , medicinal chemistry , receptor , biochemistry , medicine , paleontology , alternative medicine , pathology , biology
1,2‐Benzothiazine‐3‐carboxamide 1,1‐dioxide derivatives such as meloxicam are known to display numerous pharmacological activities. We prepared a series of 4‐hydroxy‐2‐alkyl‐2 H ‐benzo[ e ][1,2]thiazine‐3‐carboxamide 1,1‐dioxide ligands 1a – f and their Ru(η 6 ‐ p ‐cymene) complexes 2a – f , inspired by synergistic effects observed with other bioactive ligands coordinated to metal centres. The molecular structures of 1a , 2a , and 2b were determined by X‐ray diffraction analyses. The stability of the metal complexes was characterized in DMSO and DMSO/H 2 O on the basis of 1 H NMR spectroscopy and their protein binding capabilities were studied using mass spectrometry. In vitro cytotoxicities of the Ru complexes were determined against human colorectal carcinoma (HCT116), non‐small cell lung carcinoma (NCI‐H460) and cervical carcinoma (SiHa) cell lines. The low levels of biological activity observed for these Ru complexes were put into context by considering their chemical reactivity with proteins. The binding of proteins resulted in cleavage of the benzothiazine backbone when the complex was present in concentrations equimolar with respect to protein.