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Imaging Intracellular Zinc by Using a Glyoxal Bis(4‐methyl‐4‐phenyl‐3‐thiosemicarbazone) Ligand
Author(s) -
Palanimuthu Duraippandi,
Shinde Sridevi Vijay,
Dayal Disha,
Somasundaram Kumaravel,
Samuelson Ashoka G.
Publication year - 2013
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201300324
Subject(s) - chemistry , semicarbazone , glyoxal , zinc , fluorescence , ligand (biochemistry) , selectivity , flow cytometry , metal ions in aqueous solution , fluorescence microscope , metal , photochemistry , stereochemistry , biochemistry , organic chemistry , receptor , catalysis , physics , quantum mechanics , biology , genetics
The ligand glyoxal bis(4‐methyl‐4‐phenyl‐3‐thiosemicarbazone) (GTSCH 2 ) is shown to be a selective fluorescence “turn‐on” sensor for zinc ions (Zn 2+ ). This sensor is easy to synthesize, exhibits excellent sensitivity and selectivity towards Zn 2+ over other physiologically relevant cations, and has sub‐nanomolar binding affinity. It displays maximum fluorescence response to Zn 2+ when the metal/ligand ratio is 1:1 and displays stable fluorescence over a broad pH range. The potential of GTSCH 2 to image Zn 2+ inside the cell was demonstrated in MCF‐7 cells (human breast cancer cell line) by using flow cytometry and confocal fluorescence microscopy. Cell viability studies reveal that the probe is biocompatible and suitable for cellular applications.