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Synthesis, Characterization, X‐ray Structure, DNA Cleavage, and Cytotoxic Activities of Palladium(II) Complexes of 4‐Phenyl‐3‐thiosemicarbazide and Triphenylphosphane
Author(s) -
Rocha Fillipe Vieira,
Barra Carolina Valério,
Mauro Antonio Eduardo,
Carlos Iracilda Z.,
Nauton Lionel,
El Ghozzi Malika,
Gautier Arnaud,
Morel Laurent,
Netto Adelino Vieira Godoy
Publication year - 2013
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201201560
Subject(s) - chemistry , cytotoxicity , guanosine , stereochemistry , cisplatin , nuclear magnetic resonance spectroscopy , cleavage (geology) , dna , mtt assay , crystallography , in vitro , biochemistry , medicine , surgery , geotechnical engineering , chemotherapy , fracture (geology) , engineering
Abstract Complexes of the type [PdX(PPh 3 )( 1 )]X [ 1 = 4‐phenyl‐3‐thiosemicarbazide; X = Cl – ( 2 ), Br – ( 3 ), I – ( 4 ), and SCN – ( 5 )] have been synthesized and characterized by elemental analyses and IR, UV/Vis, and 1 H and 13 C NMR spectroscopy. The molecular structure of complex 4 was determined by single‐crystal X‐ray diffraction. The binding of the complexes with a purine base (guanosine) was investigated by 1 H NMR spectroscopy and mass spectrometry, which showed the complexes to coordinate to guanosine through N7. A gel electrophoresis assay demonstrated the ability of 2 – 5 to cleave DNA plasmid. All the complexes were tested in vitro by means of the MTT assay for their cytotoxicity against two murine cell lines, LM3 (mammary adenocarcinoma) and LP07 (lung adenocarcinoma), and compared with cisplatin. Complexes 2 – 5 exhibited good cytotoxicity that surpasses that of cisplatin in the case of LM3.