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Nano–Bio Interaction between Graphite Oxide Nanoparticles and Human Blood Components
Author(s) -
Kim HyoungMi,
Kim KyoungMin,
Lee Kyunghong,
Kim Yoon Suk,
Oh JaeMin
Publication year - 2012
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201200587
Subject(s) - chemistry , nanoparticle , nanomaterials , graphite oxide , human serum albumin , graphite , nanotoxicology , raman spectroscopy , nanocarriers , chemical engineering , nuclear chemistry , nanotechnology , materials science , chromatography , organic chemistry , engineering , physics , optics
We investigated the effect of graphite oxide (GO) nanoparticles, which are an emerging nanomaterial with potential applications as drug‐delivery nanocarriers, on human blood cells and plasma proteins. Graphite oxides were successfully prepared by oxidizing graphite with potassium permanganate and sulfuric acid. Powder X‐ray diffraction patterns, FTIR, and Raman spectra show that the oxidation of graphite worked well and yielded GO nanolayers. To evaluate the interaction of GO with human blood components, we studied its hemolytic effect and protein binding kinetics. The nanoparticles did not show any significant hemolytic effect during 30 min at a very high concentration of 10 mg/mL, showing high compatibility with human blood. The interaction between GO nanoparticles and human blood cells was visualized with optical microscopy. Some nanoparticles were found at the periphery of blood cells, however, significant damages or morphological changes of blood cells upon exposure to nanoparticles were not detected. When we treated GO nanoparticles with plasma proteins, such as human serum albumin, γ‐immunoglobulin, and fibrinogen, they showed concentration‐dependent binding behavior, and the binding constant is comparable with other biocompatible nanoparticles.