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Microwave‐Assisted Aqueous Synthesis of Small‐Sized, Highly Luminescent CdSeS/ZnS Core/Shell Quantum Dots for Live Cell Imaging
Author(s) -
Zhan HongJu,
Zhou PeiJiang,
He ZhenYu,
Tian Yuan
Publication year - 2012
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201200030
Subject(s) - quantum dot , luminescence , chemistry , transmission electron microscopy , nanotechnology , aqueous solution , x ray photoelectron spectroscopy , photoluminescence , fluorescence , chemical engineering , analytical chemistry (journal) , nuclear chemistry , materials science , optoelectronics , organic chemistry , optics , physics , engineering
This study develops a reliable aqueous‐phase method for the synthesis of CdSeS/ZnS core/shell QDs employing microwave irradiation technique. Firstly, CdSeS core QDs were produced by heating a precursor solution containing Cd 2+ , 3‐mercaptopropionic acid (MPA) and Na 2 SeSO 3 at 130 °C for 30 min. After purification through centrifugation, the CdSeS core QDs were successfully capped with a ZnS shell through heating a capping solution containing Zn 2+ , S 2– and MPA at 100 °C for 1 h. Both the core/shell QDs and core QDs were characterised by X‐ray powder diffraction, transmission electron microscopy and X‐ray photoelectron spectroscopy, and the epitaxial growth of a ZnS shell on the surface of CdSeS QDs was confirmed. The prepared CdSeS/ZnS core/shell QDs showed significantly enhanced luminescence and photostability than that of CdSeS core QDs. Finally, the CdSeS/ZnS core/shell QDs were applied to image live Hela cells. The pilot experiments showed that the cells were successfully stained and exhibited bright green fluorescence. The small size QDs were localised predominantly in the nuclear compartment of Hela cells with negligible cytotoxicity, even at a concentration of 300 μg mL −1 after 24 h incubation. Our results suggest that the new core/shell QDs could be highly biocompatible and have wide applications in biolabelling and imaging.