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The Zinc‐Dependent Fluorescence of a Synthetic GFP‐Like Chromophore in Organic Solvents
Author(s) -
Kang Juntao,
Fang Xinxiu,
Chen Xiankai,
Zhao Guiyan,
Ren Aimin,
Xu Jingwei,
Yang Wei
Publication year - 2011
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201100605
Subject(s) - chemistry , chromophore , fluorescence , photochemistry , zinc , green fluorescent protein , molecule , alkene , excited state , solvent , organic chemistry , biochemistry , physics , quantum mechanics , nuclear physics , gene , catalysis
A synthetic green fluorescent protein analogue showed Zn 2+ ‐induced fluorescence in organic solvents ( Φ = 0.07 at r.t. and up to 0.3 at low temperature). The experimental observations and theoretical calculations suggest that Zn 2+ binding inhibits the free rotation of an aryl–alkene bond, which allows excited molecules to relax through the emission pathway instead of the thermodynamic pathway. The solvent influenced the fluorescence intensities and wavelengths by direct or indirect interactions with the molecules.