Premium
Luminescent Hybrids Combining a Metal Complex and a Crown Ether – Receptors for Peptidic Ammonium Phosphates
Author(s) -
Späth Andreas,
Koch Carina,
König Burkhard
Publication year - 2010
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.201000239
Subject(s) - chemistry , crown ether , aqueous solution , intramolecular force , metal ions in aqueous solution , ether , fluorophore , ammonium , metal , luminescence , receptor , inorganic chemistry , stereochemistry , organic chemistry , ion , fluorescence , biochemistry , physics , optoelectronics , quantum mechanics
Hybrid compounds combining a luminescent aza crown ether and 1,4,7,10‐tetraazacyclododecane (cyclene) or bis(pyridin‐2‐ylmethylamine) (bpa) metal complexes were prepared. These synthetic receptors signal the presence of peptidic ammonium phosphates in buffered aqueous solution by changes in their emission intensity. Reversible phosphate ion coordination to the metal complex binding sites decreases the emission intensity of the phthalic ester fluorophore. Structurally suitable ammonium phosphates, such as the C‐terminal domain of RNA polymerase II or the protein kinase G target sequence, bind to the synthetic receptors by an additional intramolecular interaction between the ammonium ion and the aza crown ether. The binding of the ammonium ion to the aza crown ether increases the emission intensity of the fluorophore. In aqueous solution, this weak interaction can only occur within aggregates of phosphates and metal complex receptors of matching geometry;therefore, it allows the distinction of structurally related phosphorylated peptides in buffered aqueous solution.