Premium
Synthesis, Radiolabelling and Confocal Fluorescence Microscopy of Styrene‐Derivatised Bis(thiosemicarbazonato)zinc and ‐copper Complexes
Author(s) -
Holland Jason P.,
Barnard Peter J.,
Bayly Simon R.,
Betts Helen M.,
Churchill Grant C.,
Dilworth Jonathan R.,
Edge Ruth,
Green Jennifer C.,
Hueting Rebekka
Publication year - 2008
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.200701351
Subject(s) - chemistry , copper , zinc , fluorescence , electron paramagnetic resonance , photochemistry , fluorescence spectroscopy , cyclic voltammetry , electrochemistry , styrene , analytical chemistry (journal) , electrode , organic chemistry , nuclear magnetic resonance , physics , quantum mechanics , copolymer , polymer
The synthesis of zinc(II) and copper(II) complexes of an unsymmetrical bis(thiosemicarbazonato) ligand containing a reactive styrene group are reported. The compounds have been characterised by a range of techniques including reverse‐phase HPLC, cyclic voltammetry, NMR, UV/Vis, electron paramagnetic resonance and fluorescence emission spectroscopy. Time‐dependent density functional theory calculations have been used to assign the electronic absorption spectrum of [Zn II ATSM] and probe the nature of the fluorescent excited state. Electrochemistry experiments show that the copper(II) complex undergoes quasi‐reversible one‐electron reduction at biologically accessible potentials and is within the range proposed for the complex to be hypoxia‐selective. The copper‐64 radiolabelled complex has been prepared in aqueous solution and characterised by reverse‐phase radio‐HPLC. Cellular uptake in HeLa cells has been observed using confocal fluorescence microscopy.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)