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Polypeptide Adsorption on a Synthetic Montmorillonite: A Combined Solid‐State NMR Spectroscopy, X‐ray Diffraction, Thermal Analysis and N 2 Adsorption Study
Author(s) -
Gougeon Régis D.,
Soulard Michel,
Reinholdt Marc,
MiehéBrendlé Jocelyne,
Chézeau JeanMichel,
Dred Ronan Le,
Marchal Richard,
Jeandet Philippe
Publication year - 2003
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.200390177
Subject(s) - chemistry , montmorillonite , adsorption , random coil , crystallography , silicate , nuclear magnetic resonance spectroscopy , nmr spectra database , x ray crystallography , solid state nuclear magnetic resonance , diffraction , stereochemistry , organic chemistry , spectral line , circular dichroism , nuclear magnetic resonance , astronomy , physics , optics
Two homopolypeptides, polylysine and polyglutamic acid, were adsorbed on a synthetic montmorillonite clay, in acidic medium. These organic‐inorganic complexes can be heated up to 110 °C without any degradation of the polypeptide. 1 H‐ 13 C CP‐MAS NMR spectra show that these polypeptides, which exhibit a mixture of α‐helical and random coil conformations in the bulk, tend to unfold and adopt a more extended random coil structure on adsorption on the phyllosilicate. The values of the basal spacing measured by X‐ray diffraction on dehydrated samples clearly indicate that in the presence of adsorbed polypeptide, the silicate layers do not collapse. The incorporation of polypeptide fragments within the interlayer space is also revealed by the decrease in the specific surface areas measured by N 2 ‐adsorption BET experiments. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2003)