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miR‐191 modulates B‐cell development and targets transcription factors E2A, Foxp1, and Egr1
Author(s) -
Blume Jonas,
Ziętara Natalia,
Witzlau Katrin,
Liu Yanshan,
Sanchez Oskar Ortiz,
Puchałka Jacek,
Winter Samantha J.,
KunzeSchumacher Heike,
Saran Namita,
Düber Sandra,
Roy Bishnudeo,
Weiss Siegfried,
Klein Christoph,
Wurst Wolfgang,
Łyszkiewicz Marcin,
Krueger Andreas
Publication year - 2019
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.201847660
Subject(s) - biology , egr1 , ectopic expression , transcription factor , microrna , microbiology and biotechnology , somatic cell , transcriptional regulation , gene , transcription (linguistics) , genetics , linguistics , philosophy
Abstract The interdependence of posttranscriptional gene regulation via miRNA and transcriptional regulatory networks in lymphocyte development is poorly understood. Here, we identified miR‐191 as direct upstream modulator of a transcriptional module comprising the transcription factors Foxp1, E2A, and Egr1. Deletion as well as ectopic expression of miR‐191 resulted in developmental arrest in B lineage cells, indicating that fine tuning of the combined expression levels of Foxp1, E2A, and Egr1, which in turn control somatic recombination and cytokine‐driven expansion, constitutes a prerequisite for efficient B‐cell development. In conclusion, we propose that miR‐191 acts as a rheostat in B‐cell development by fine tuning a key transcriptional program.