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Expansion of CD 16 positive and negative human NK cells in response to tumor stimulation
Author(s) -
Tsukerman Pinchas,
SternGinossar Noam,
Yamin Rachel,
Ophir Yael,
Stanietsky Anna Miller, Noa,
Mandelboim Ofer
Publication year - 2014
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.201344170
Subject(s) - biology , cd16 , microbiology and biotechnology , receptor , interleukin 21 , lymphokine activated killer cell , nk 92 , interleukin 12 , cell culture , population , janus kinase 3 , immune system , cell , immunology , t cell , cytotoxic t cell , in vitro , cd8 , cd3 , biochemistry , medicine , genetics , environmental health
NK cells are innate immune lymphocytes that express a vast repertoire of germ‐line encoded receptors for target recognition. These receptors include inhibitory and activating proteins, among the latter of which is CD 16, a low affinity binding F c receptor. Here, we show that human NK cells expand in response to stimulation with various tumor cell lines. We further demonstrate that the tumor‐derived expansion of NK cells is accompanied by rapid, cell‐dependent, changes in CD 16 expression levels. We show that in NK cells expanded in response to the EBV ‐transformed cell line 721.221, CD 16 is shed and therefore approximately half of the expanded 721.221‐derived NK ‐cell population does not express CD 16. We also show, in contrast, that in response to 1106mel cells, CD 16 expression is maintained on the cell surface of the expanded NK cells due to an antibody‐dependent mechanism. Our results may provide a basis for the selective expansion of NK cells that may be used for tumor immunotherapy.