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IL ‐13 R α1 is a surface marker for M 2 macrophages influencing their differentiation and function
Author(s) -
Dhakal Mermagya,
Hardaway John C.,
Guloglu Fatma Betul,
Miller Mindy M.,
Hoeman Christine M.,
Zaghouani Adam A.,
Wan Xiaoxiao,
Rowland Linda M.,
Cascio Jason A.,
Sherman Michael P.,
Zaghouani Habib
Publication year - 2014
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.201343755
Subject(s) - biology , phenotype , microbiology and biotechnology , cytokine , cellular differentiation , immunology , biochemistry , gene
In this study, we examined the role IL ‐13 receptor alpha 1 ( IL ‐13 R α1) plays in macrophage differentiation and function. The findings indicate that IL ‐13 R α1 is expressed on the M 2 but not on the M 1 subset of macrophages and specifically heterodimerizes with the IL ‐4 R α chain to form a type II receptor, which controls the differentiation and function of these cells. Indeed, BM cells from IL ‐13 R α1 +/+ and IL ‐13 R α1 −/− mice yield equivalent numbers of macrophages when cultured under M 2 polarizing conditions. However, IL ‐13 R α1 −/− BM cells yield a much higher number of macrophages than IL ‐13 R α1 +/+ BM cells when the differentiation is carried out under M 1‐polarizing conditions. Further analyses indicated that macrophages that express IL ‐13 R α1 also display surface markers associated with an M 2 phenotype. In addition, the IL ‐13 R α1 + macrophages were highly efficient in phagocytizing zymosan bioparticles both in vitro and in vivo, and supported differentiation of naïve T cells to a T h2 phenotype. Finally, when stimulated by IL ‐13, a cytokine that uses the heteroreceptor, the cells were able to phosphorylate STAT 6 efficiently. These previously unrecognized findings indicate that IL ‐13 R α1 serves as a marker for M 2 macrophages and the resulting heteroreceptor influences both their differentiation and function.