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UXT is a novel regulatory factor of regulatory T cells associated with F oxp3
Author(s) -
Li Weina,
Wang Lili,
Jiang Changli,
Li Hong,
Zhang Kuo,
Xu Yujin,
Hao Qiang,
Li Meng,
Xue Xiaochang,
Qin Xin,
Zhang Cun,
Wang Huixuan,
Zhang Wei,
Zhang Yingqi
Publication year - 2014
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.201343394
Subject(s) - gene knockdown , foxp3 , regulator , microbiology and biotechnology , phenotype , regulatory t cell , chemistry , biology , immune system , gene , t cell , biochemistry , immunology , il 2 receptor
Regulatory T ( T reg) cells are a constitutively immunosuppressive subtype of T cells that contribute to the maintenance of immunological self‐tolerance and immune homeostasis. However, the molecular mechanisms involved in the regulation of T reg cells remain unclear. In the present study, we identified ubiquitously expressed transcript ( UXT ) to be a novel regulator of human T reg‐cell function. In cultured human T reg cells, UXT associates with F oxp3 in the nucleus by interacting with the proline‐rich domain in the N ‐terminus of F oxp3. Knockdown of UXT expression in T reg cells results in a less‐suppressive phenotype, demonstrating that UXT is an important regulator of the suppressive actions of T reg cells. Depletion of UXT affects the localization stability of F oxp3 protein in the nucleus and downregulates the expression of F oxp3‐related genes. Overall, our results show that UXT is a cofactor of F oxp3 and an important player in T reg‐cell function.