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Targeting antigen to bone marrow stromal cell‐2 expressed by conventional and plasmacytoid dendritic cells elicits efficient antigen presentation
Author(s) -
Moffat Jessica M.,
Segura Elodie,
Khoury Gabriela,
Caminschi Irene,
Cameron Paul U.,
Lewin Sharon R.,
Villadangos Jose A.,
Mintern Justine D.
Publication year - 2013
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.201242799
Subject(s) - antigen presentation , biology , antigen , antigen processing , dendritic cell , antigen presenting cell , mhc class i , microbiology and biotechnology , stromal cell , immunology , mhc class ii , cross presentation , cd8 , t cell , bone marrow , major histocompatibility complex , immune system , cancer research
Bone marrow stromal cell‐2 ( BST ‐2) has major roles in viral tethering and modulation of interferon production. Here we investigate BST ‐2 as a receptor for the delivery of antigen to dendritic cells ( DC s). We show that BST ‐2 is expressed by a panel of mouse and human DC subsets, particularly under inflammatory conditions. The outcome of delivering antigen to BST ‐2 expressed by steady state and activated plasmacytoid DC (p DC ) or conventional CD 8 + and CD 8 − DC s was determined. T ‐cell responses were measured for both MHC class I ( MHCI ) and MHC class II ( MHCII ) antigen presentation pathways in vitro. Delivering antigen via BST ‐2 was compared with that via receptors DEC 205 or S iglec‐ H . We show that despite a higher antigen load and faster receptor internalisation, when antigen is delivered to steady state or activated p DC via BST ‐2, BST ‐2‐targeted activated conventional DC s present antigen more efficiently. Relative to DEC 205, BST ‐2 was inferior in its capacity to deliver antigen to the MHCI cross‐presentation pathway. In contrast, BST ‐2 was superior to S iglec‐ H at initiating either MHCI or MHCII antigen presentation. In summary, BST ‐2 is a useful receptor to target with antigen, given its broad expression pattern and ability to access both MHCI and MHCII presentation pathways with relative efficiency.