Premium
ZNF 385 B is characteristically expressed in germinal center B cells and involved in B ‐cell apoptosis
Author(s) -
Iijima Kazutoshi,
Yamada Hiroyuki,
Miharu Masashi,
Imadome KenIchi,
Miyagawa Yoshitaka,
Akimoto Shingo,
Kobayashi Kenichiro,
Okita Hajime,
Nakazawa Atsuko,
Fujiwara Shigeyoshi,
Fujimoto Junichiro,
Kiyokawa Nobutaka
Publication year - 2012
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.201242530
Subject(s) - biology , microbiology and biotechnology , apoptosis , b cell , downregulation and upregulation , transactivation , germinal center , ectopic expression , gene isoform , cell culture , transcription factor , antibody , gene , immunology , genetics
We previously identified zinc finger ( ZF ) protein ZNF 385 B as a molecule specifically expressed in B urkitt's lymphoma ( BL ) among hematologic malignancies. Here, we investigated ZNF 385 B expression in healthy B cells in a variety of hematological tissues by RT ‐ PCR and immunohistochemistry. ZNF 385 B expression was found to be limited to a subset of GC B cells, the healthy counterpart to BL B cells. To elucidate the function of ZNF 385 B in healthy B cells, we established a tetracycline‐controlled protein‐inducible system in B ‐cell lines and observed that ectopic expression of the longest transcript variant of ZNF 385 B , possessing four ZF domains, induced upregulation of PERP and FAS / CD 95 , a downstream target of p53, and activation of caspase, resulting in apoptosis induction. However, a ZNF 385 B deletion mutant with three ZF domains corresponding to shorter isoforms, did not induce upregulation; rather it inhibited apoptosis induced by CD 20 cross‐linking and BCR stimulation. The direct binding of ZNF 385 B with p53 has suggested the involvement of ZNF 385 B in B ‐cell apoptosis via modulation of p53 transactivation; our data indicate that ZNF 385 B characteristically expressed in GC B cells has both proapoptotic and antiapoptotic activities depending on the type of isoform and should be a novel player in GC B ‐cell selection.