Premium
Loss of E protein transcription factors E 2 A and HEB delays memory‐precursor formation during the CD 8 + T ‐cell immune response
Author(s) -
D'Cruz Louise M.,
Lind Kristin Camfield,
Wu Bei Bei,
Fujimoto Jessica K.,
Goldrath Ananda W.
Publication year - 2012
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.201242497
Subject(s) - biology , cytotoxic t cell , effector , cd8 , immune system , microbiology and biotechnology , transcription factor , t cell , t cell receptor , immunology , genetics , gene , in vitro
The transcription factors E 2 A and HEB (members of the E protein family) have been shown to play essential roles in lymphocyte development, while their negative regulators, the I d proteins, have been implicated in both lymphocyte development and in the CD 8 + T ‐cell immune response. Here, we show that E proteins also influence CD 8 + T cells responding to infection. E protein expression was upregulated by CD 8 + T cells during the early stages of infection and increased E protein DNA ‐binding activity could be detected upon TCR stimulation. Deficiency in the E proteins, E 2 A and HEB , led to increased frequency of terminally differentiated effector KLRG 1 hi CD 8 + T cells in mice during infection, and decreased generation of longer‐lived memory‐precursor cells during the immune response. These data suggest a model whereby E protein transcription factor activity favors rapid memory‐precursor T ‐cell formation while their negative regulators, I d2 and I d3, are both required for robust effector CD 8 + T ‐cell response during infection.