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The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion
Author(s) -
Lax Siân,
Hardie Debbie L.,
Wilson Amy,
Douglas Mike R.,
Anderson Graham,
Huso David,
Isacke Clare M.,
Buckley Christopher D.
Publication year - 2010
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.200939877
Subject(s) - biology , stromal cell , population , immune system , pericyte , lymph node , microbiology and biotechnology , phenotype , immunology , pathology , cancer research , in vitro , gene , genetics , endothelial stem cell , medicine , demography , sociology
Abstract CD248 is a cell surface receptor that specifically identifies fibroblasts and pericytes during development and in association with cancer and inflammation. However, its function is poorly defined and its role in lymphoid organs not studied. Here, we used (4‐hydroxy‐3‐nitrophenyl)acetyl chicken γ‐globulin immunisation and mice lacking CD248 to study whether CD248 modulates popliteal LN (pLN) expansion and subsequent immune responses. We have found that CD248 is required for complete pLN expansion but not for co‐ordination of B and T cell compartmentalisation or antibody production following (4‐hydroxy‐3‐nitrophenyl)acetyl chicken γ‐globulin immunisation. In vitro , we show that CD248 expression in human MG63 stromal cells and mouse embryonic fibroblasts leads to a pro‐proliferative and pro‐migratory phenotype. This correlates with a proliferating CD248 + population observed in vivo during pLN expansion. Taken together, these data highlight a role for CD248 in secondary lymphoid organ remodelling during adaptive immune responses.