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RelB/p50 regulates CCL19 production, but fails to promote human DC maturation
Author(s) -
Gasparini Chiara,
Foxwell Brian M. J.,
Feldmann Marc
Publication year - 2009
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.200939209
Subject(s) - relb , ccl19 , microbiology and biotechnology , p50 , biology , chemokine , immunology , immune system , chemokine receptor , biochemistry , transcription factor , gene
Abstract DC, when fully matured are the APC best able to activate naïve T cells. Recently, we demonstrated using adenoviruses overexpressing IκBα and proteosome inhibitors that NF‐κB is involved in DC activation, but the role of the individual subunits is still not clear. We investigated the role of the NF‐κB subunits RelB and p50 in human DC activation using adenoviral vectors expressing RelB or p50. Nuclear RelB, in the form of RelB/p50, was active only in DC infected with both viruses, this induced the production of the soluble homeostatic chemokine CCL19, but not other homeostatic chemokines, particularly in LPS‐matured DC. However, RelB/p50 did not affect the expression of costimulatory and antigen‐presenting molecules, and increased the allogeneic mixed lymphocyte reaction only in LPS‐matured DC. This enhanced mixed lymphocyte reaction is most likely due to enhanced CCL19 production, which sustains the interaction between mature DC and naïve T cells. In conclusion, we demonstrated that RelB/p50 was active only in DC expressing both RelB and p50, and induced CCL19 production, but not DC maturation.