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WC1 + γδ T cell memory population is induced by killed bacterial vaccine
Author(s) -
Blumerman Seth L.,
Herzig Carolyn T. A.,
Baldwin Cynthia L.
Publication year - 2007
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.200636216
Subject(s) - priming (agriculture) , antigen , biology , il 2 receptor , t cell , population , immunology , memory t cell , cd40 , naive t cell , leptospira , interleukin 21 , cytotoxic t cell , immune system , cd8 , in vitro , t cell receptor , medicine , germination , environmental health , serotype , biochemistry , botany
Abstract Limited studies have addressed the ability of γδ T cells to become memory populations. We previously demonstrated that WC1.1 + γδ T cells from ruminants vaccinated with killed Leptospira borgpetersenii proliferate and produce IFN‐γ in recall responses. Here we show that this response is dependent upon antigen‐responsive CD4 T cells, at least across transwell membranes; this requirement cannot be replaced by IL‐2. The response was also dependent upon in vivo priming, since γδ T cells from leptospira vaccine‐naive animals did not respond to antigen even when co‐cultured across membranes from antigen‐responsive PBMC. γδ T cells were the major antigen‐responding T cell population for the first 4 wks following vaccination and replicated more rapidly than CD4 T cells. Primed WC1 + γδ T cells circulated as CD62L hi /CD45RO int /CD44 lo , characteristics of T CM cells. When stimulated with antigen, they decreased CD62L, increased CD44 and CD25, and had no change in CD45RO expression. These changes paralleled those of the leptospira antigen‐responsive CD4 T cells but differed from those of γδ T cells proliferating to mitogen stimulation. This system for in vivo γδ T cell priming is unique, since it relies on a killed antigen to induce memory and may be pertinent to designing vaccines that require type 1 pro‐inflammatory cytokines.