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Unexpected features of acute T lymphoblastic lymphomas in Notch1IC transgenic rats
Author(s) -
van den Brandt Jens,
Kwon SoonHwan,
McPherson Kirsty G.,
Petrovic Suzana,
Zettl Andreas,
MüllerHermelink Hans Konrad,
Reichardt Holger M.
Publication year - 2006
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.200535791
Subject(s) - biology , lymphoma , transgene , notch signaling pathway , pathogenesis , genetically modified mouse , cd8 , cancer research , bone marrow , phenotype , bcl10 , double negative , immunology , t cell , signal transduction , microbiology and biotechnology , gene , antigen , immune system , genetics
Abstract Dysregulated Notch signaling accounts for the majority of acute T lymphoblastic leukemia/lymphoma (T‐ALL) cases in humans. Here, we characterize lymphomas from Notch1IC transgenic rats, which develop T‐ALL shortly after weaning, and show that they display a number of previously undocumented features. Starting from monoclonal thymic tumors, the CD4 + CD8αα + lymphoma cells infiltrate the bone marrow and then spread to secondary lymphoid and non‐lymphoid organs. However, major hallmarks of T‐ALL cells in other murine models and human patients, such as constitutive NF‐κB activity and increased levels of anti‐apoptotic proteins, are remarkably absent in Notch1IC lymphomas. In contrast, CD30, a classic marker of Hodgkin lymphomas, is overexpressed in these tumors. Intriguingly, enforced Notch1 signaling up‐regulates expression of Notch3, which has also been implicated in the pathogenesis of T‐ALL. By blocking endogenous Notch signaling, we could demonstrate that Notch1IC is sufficient to induce sustained preTCR expression in transgenic thymocytes but not for their progression to the double‐positive stage. This suggests that other Notch activities may also contribute to the phenotype of the transgenic rats. In summary, we anticipate this new animal model will help to further elucidate the role of Notch1 in the pathogenesis of T‐ALL.