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Impaired maturation and function of dendritic cells by mycobacteria through IL‐1β
Author(s) -
Makino Masahiko,
Maeda Yumi,
Mukai Tetsu,
Kaufmann Stefan H. E.
Publication year - 2006
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.200535727
Subject(s) - biology , immune system , innate immune system , immunology , acquired immune system , dendritic cell , lipopolysaccharide , cd86 , antigen , antigen presentation , microbiology and biotechnology , interleukin 12 , t cell , cytotoxic t cell , in vitro , biochemistry
Abstract Dendritic cells (DC) are pivotal for initiation and regulation of innate and adaptive immune responses evoked by vaccination and natural infection. After infection, mycobacterial pathogens first encounter monocytes, which produce pro‐inflammatory cytokines, including IL‐1β, TNF‐α and IL‐6. The role of these cytokines in DC maturation remains incompletely understood. Here, we show that maturation of DC from monocytes was impaired by pretreatment of monocytes with low doses of IL‐1β. Under these conditions, Mycobacterium leprae ‐infected DC failed to stimulate antigen‐specific T cell responses. Expression of CD86 and CD83 and production of IL‐12 in response to lipopolysaccharide and peptidoglycan were diminished. In contrast, these DC functions were not impaired by pretreatment with TNF‐α, IL‐6 or IL‐10. When monocytes were infected with M. bovis Bacillus Calmette‐Guérin, and subsequently differentiated to DC, the activity of these DC was suppressed as well. Thus, IL‐1β acts at early stages of differentiation of DC and impairs biological functions of DC at later stages. Therefore, production of IL‐1β by mycobacteria‐infected antigen‐presenting cells counteracts effective stimulation of innate and adaptive immune responses.

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