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NK cells contribute to the early clearance of HSV‐1 from the lung but cannot control replication in the central nervous system following intranasal infection
Author(s) -
Reading Patrick C.,
Whitney Paul G.,
Barr Daniel P.,
Smyth Mark J.,
Brooks Andrew G.
Publication year - 2006
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.200535710
Subject(s) - biology , herpes simplex virus , immunology , virus , clearance , viral replication , virology , cytotoxic t cell , immune system , nasal administration , central nervous system , immunity , respiratory tract , innate immune system , lung , respiratory system , medicine , neuroscience , biochemistry , anatomy , in vitro , urology
Abstract While infection of the respiratory tract with herpes simplex virus type 1 (HSV‐1) can have severe clinical complications, little is known of the immune mechanisms that control both the replication and spread of HSV‐1 in this site. To better understand the contribution of innate immunity and in particular natural killer (NK) cells to the control of infection at this site, we have utilized a mouse model of intranasal HSV‐1 infection. NK cell numbers increased in the lung following intranasal infection and they produced IFN‐γ and acquired an enhanced cytotoxic capacity. While depletion of NK cells resulted in increased HSV‐1 titres in the lung, the time taken to clear the virus was unaffected. Interestingly, HSV‐1 was also effectively cleared from the lungs of RAG‐1 –/– mice that lack both B and T cells. However, RAG‐1 –/– mice could not control the spread of virus to the central nervous system and its subsequent replication in the brain. Together, these data demonstrate that NK cells are recruited, activated and contribute to early protection of the lung during acute HSV‐1 infection of the respiratory tract, but in the absence of adaptive immunity are unable to control the replication and spread of virus in the nervous system.