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Responses to the soluble flagellar protein FliC are Th2, while those to FliC on Salmonella are Th1
Author(s) -
Cunningham Adam F.,
Khan Mahmood,
Ball Jennifer,
Toellner KaiMichael,
Serre Karine,
Mohr Elodie,
MacLennan Ian C. M.
Publication year - 2004
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.200425403
Subject(s) - biology , priming (agriculture) , recombinant dna , context (archaeology) , messenger rna , interleukin 4 , cytokine , phenotype , antigen , microbiology and biotechnology , gene , genetics , paleontology , botany , germination
Abstract Features of the Th1 or Th2 phenotype start to develop during CD4 T cell priming. This study of the response to the bacterial flagellar protein FliC shows that either Th1 or Th2 responses can be induced in mice depending upon how FliC is presented. This is shown by assessing the cytokine mRNA and class of FliC‐specific plasma cells induced in situ . Soluble recombinant (r)FliC and polymerized FliC are strongly Th2 polarizing, inducing IL‐4, NIP45 and c‐Maf mRNA as well as ϵ and γ1 switch transcripts and switching to IgG1. CD28‐requirement for this switching shows its T cell dependence. rFliC was unable to induce markers of Th1 activity including IL‐12, T‐bet and IFN‐γ. Conversely, when FliC is presented in its native context surface‐bound on live, flagellated Salmonella, switching is predominantly to IgG2a (IgG2c in C57BL/6 mice), reflecting Th1 activity. The development of divergent FliC‐specific polarization to either Th1 or Th2 indicates that the context in which this antigen is encountered rather than its intrinsic immunostimulatory properties determines the direction of Th polarization.