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Early cytoskeletal rearrangement during dendritic cell maturation enhances synapse formation and Ca 2+ signaling in CD8 + T cells
Author(s) -
Averbeck Marco,
Braun Thorsten,
Pfeifer Gunther,
Sleeman Jonathan,
Dudda Jan,
Martin Stefan F.,
Kremer Bernhard,
Aktories Klaus,
Simon Jan C.,
Termeer Christian
Publication year - 2004
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.200425355
Subject(s) - immunological synapse , microbiology and biotechnology , t cell , biology , cytoskeleton , cytotoxic t cell , jurkat cells , cd28 , t cell receptor , cd8 , confocal microscopy , antigen presenting cell , biophysics , cell , immunology , antigen , immune system , biochemistry , in vitro
Abstract The interplay between dendritic cells (DC) and T cells is a dynamic process critically depending on DC maturation. Ca 2+ influx is one of the initial events occurring during DC/T cell contacts. To determine how DC maturation influences DC/T cell contacts, time‐lapse video microscopy was established using TCR‐transgenic CD8 + T cells from P14 mice. DC maturation shifted DC/T cell contacts from short‐lived interactions with transient Ca 2+ influx in T cells to long‐lasting interactions and sustained Ca 2+ influx of 30 min and more. Follow‐up of DC/T cell interactions after 2 h using confocal microscopy revealed that long‐lasting Ca 2+ responses in T cells were preferentially associated with the formation of an immunological synapse involving CD54 and H2‐K b at the DC/T cell interface. Such synapse formation preceded MHC or B7 up‐regulation, since DC developed into potent Ca 2+ stimulators 7 h after initiation of maturation. Instead, the enhanced capacity of 7 h‐matured DC to induce sustained Ca 2+ responses in CD8 + T cells is critically dependent on the polarization and rearrangement of the cytoskeleton, as shown by Clostridium difficile toxin B inhibitor experiments. These data indicate that already very early after receiving a maturation stimulus, DC display enhanced cytoskeletal activity resulting in the rapid formation of immunological synapses and effective CD8 + T cell stimulation.