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Exposure to myeloma cell lysates affects the immune competence of dendritic cells and favors the induction of Tr1‐like regulatory T cells
Author(s) -
Fiore Francesca,
Nuschak Barbara,
Peola Silvia,
Mariani Sara,
Muraro Michela,
Foglietta Myriam,
Coscia Marta,
Bruno Benedetto,
Boccadoro Mario,
Massaia Massimo
Publication year - 2005
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.200425093
Subject(s) - biology , immune system , microbiology and biotechnology , immunology , multiple myeloma , dendritic cell , cell , genetics
Abstract The aim of this work was to investigate the interactions of tumor cells with dendritic cells (DC) in normal donors and patients with multiple myeloma (MM). Normal and MM DC internalized necrotic lysates derived from myeloma cell lines (MCL) with high efficiency, whereas necrotic lysates from primary myeloma cells (PMC) were internalized with significantly lower efficiency. A positive correlation was found between susceptibility to internalization and the ability to induce DC maturation. After PMC exposure, DC produced large amounts of IL‐10 and measurable amounts of IL‐4 but no detectable IL‐12. Two rounds of exposure to PMC‐treated DC generated autologous T cells with low proliferative capacity, decreased IFN‐γ production and increased IL‐10 production in the absence of IL‐4 production. These data indicate that myeloma cells can affect host immunity by priming DC towards a maturation state favoring the generation of T cells with a regulatory rather than an effector phenotype.