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A murine transmembrane tumor necrosis factor (TNF) transgene induces arthritis by cooperative p55/p75 TNF receptor signaling
Author(s) -
Alexopoulou Lena,
Pasparakis Manolis,
Kollias George
Publication year - 1997
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830271018
Subject(s) - tumor necrosis factor alpha , transmembrane protein , biology , transgene , arthritis , cytokine , gene knockout , receptor , in vivo , transmembrane domain , genetically modified mouse , microbiology and biotechnology , knockout mouse , immunology , cancer research , gene , biochemistry , genetics
Abstract The arthritogenic activities of tumor necrosis factor (TNF) and its p55TNF‐receptor (R) have been well documented in experimental animal models of arthritis, and in transgenic mice expressing wild‐type or mutant transmembrane human TNF proteins in their joints. In this study we show that chronic inflammatory arthritis also develops in transgenic mice made to overexpress a mutant transmembrane from of the murine TNF protein (muTNF Δ1–12 ) which is known to utilize efficiently both the p55 and the p75TNFR. Cross‐breeding of the transgene into a TNF knockout background did not alter development of disease. Analysis of TNF bioactivity in sera from lipopolysaccharide‐stimulated mice or ex vivo macrophage cultures demonstrated that the muTNF Δ1–12 protein accumulates on the cell surface and is not processed to bioactive soluble TNF, indicating that transmembrane TNF is by itself sufficient to mediate pathogenesis of arthritis. Furthermore, using TNFR knockout mice, it is shown that development of transmembrane TNF‐mediated arthritis requires the presence of the p55TNFR but is significantly delayed in the absence of the p75TNFR, suggesting a positive cooperation between the two TNFR in the arthritogenic process. These results indicate that blocking the activities of both soluble and transmembrane TNF may be required to effectively neutralize the pathogenic potential of this cytokine in arthritis.